Oleksandr Kukharchuk
ReeLabs Pvt. Ltd, India
Title: Modeling of the organ-specific niche of stem cells as a necessary condition for increasing the effectiveness of stem cell treatment
Biography
Biography: Oleksandr Kukharchuk
Abstract
The interactions between stem cells (SCs) and their surrounding microenvironment are pivotal to determine tissue homeostasis and SCs renewal or differentiation in vivo. One of the way for modulation of organ-specific niches for SCs transplantation could be established by using fetal tissue extracts of organs obtained from the stage of incomplete organogenesis, then microenvironment in each of the organs must be specific and enough to generate constant signal for the final SCs differentiation. The objective was to recreate the natural niche microenvironment during cell therapy. We investigated the concentrations of growth factors in tissue extracts of various fetal organs, and studied the efficacy of fetal tissue extracts (FTEs) in patients with non-healing wounds/ulcer who did not respond to previous stem cells treatment. The level of growth factors in the skin, lung, liver, heart, brain, lung and kidney differs considerably. This may be due to differences in regulatory niches that are specific to each organ, which determines the direction of differentiation of the fetal SCs. Treatment included intravenous SCs administration; local multiple injections of SCs along obliterated arteries, around the ulcer, and in the bottom of the wound; and application of FTEs (liver, skin, and muscle) in auto plasma gel. Laser Doppler flow-metry showed the significant improvement of the micro-hemodynamic just 1 month after last administration of FTEs. Selective X-ray contrast arteriography showed the increase of area and expansion of the collateral arterial vasculature. Angiogenesis activation was proved by electronic microscopy, histologically and by immunohistochemistry. Wounds/ulcers have good epithelization and complete healing.