Shagidulin Murat
I.M. Sechenov First Moscow State Medical University, Russia
Title: Usage of tissue engineering for stimulation reparative processes in damaged liver
Biography
Biography: Shagidulin Murat
Abstract
Background: Tissue engineering and cell therapy is a new perspective method for recovery function and the formation of new centers of regeneration of damaged organs.
Aim: Creating technology for the treatment of chronic liver failure (CLF) by growing the functionally active liver tissue and its regeneration.
Methods: (CLF) was modeled on Wistar rats by means of ССl4. Mesenchymal stromal cells of bone marrow (MSC) were obtained from August rats by standard procedure. MSC (0, 5-0,8х106cells/cm3) was cultivated for 10 days and then applied on the composition of heterogenic implantable hydrogel. Formed cell engineering constructions (CECs) were implanted into damaged rat liver. The animals were divided into 2 groups: control gr.1 (n=25) without treatment-injected saline and with treatment-gr.2 (n=25). Dynamics reduction of CLF; liver and CECs morphology were investigated within 365 days after implantation.
Results: In gr.1 took place mortality (48%), the formation of liver cirrhosis without recovery. In gr.2 all biochemical indices returned to normal levels within 30-60. On 90 days it was shown restoration of a hepatic lobe structure and liver architectonics, viable and proliferative activity of MSC. Also detected neogenic plethoric vessels and neogenic bile ducts were detected.
Conclusion: Studies found out that the proposed method of implantation CECs with MSC is effective method for stimulation of reparative regeneration in the damaged liver, treatment CLF and can be used in clinical practice.