14^th International Conference on ^{Tissue Engineering & Regenerative Medicine} April 25-26, 2019 Amsterdam, Netherlands

Biography:

José Manuel Baena, research associate "Advanced therapies: differentiation, regeneration and cancer" IBIMER,CIBM, Universidad de Granada. Founder of BRECA Health Care, pioneer in 3D printed custom made implants for orthopedic surgery, and REGEMAT 3D, the first Spanish bioprinting company. Expert in innovation, business development and internationalization, lecturer in some business schools, he is passionate about biomedicine and technology. In his free time he is also researcher at the Biopathology and Regenerative Medicine Institute (IBIMER).

Abstract:

Tissue regeneration (TR) is currently one of the most challenging biotechnology unsolved problems. Tissue engineering (TE) is a multidisciplinary science that aims at solving the problems of TR. TE could solve pathologies and improve the quality of life of billions of people around the world suffering from tissue damages. New advances in stem cell (SC) research for the regeneration of tissue injuries have opened a new promising research field. However, research carried out nowadays with two-dimensional (2D) cell cultures do not provide the expected results, as 2D cultures do not mimic the 3D structure of a living tissue. Some of the commonly used polymers for cartilage regeneration are Poly-lactic acid (PLA) and its derivates as Poly-L-lactic acid (PLLA), Poly(glycolic acids) (PGAs) and derivates as Poly(lactic-co-glycolic acids) (PLGAs) and Poly caprolactone (PCL). All these materials can be printed using fused deposition modelling (FDM), a process in which a heated nozzle melt a thermoplastic filament and deposit it in a surface, drawing the outline and the internal filling of every layer. All this procedures uses melting temperatures that decrease viability and cell survival. Research groups around the world are focusing their efforts in finding low temperature printing thermoplastics or restricted geometries that avoid the contact of the thermoplastic and cells at a higher temperature than the physiologically viable. This has mainly 2 problems; new biomaterials need a long procedure of clearance before they can be used in clinical used, and restrictions in geometries will limit the clinical application of 3D printing in TE.

Biography:

Joseph Choukroun has completed his MD in Anesthesiologist from the University of Montpellier, France. He is a Specialist in Pain therapy at University of Strasbourg, France. He is the Inventor of the PRF® technique (Platelet Rich Fibrin). He is the President of the SYFAC: International symposium on growth factors. He is a Researcher and recognized as international Speaker.

Abstract:

Regenerative therapy with stem cells has gained tremendous momentum over the past decade as a modality geared towards markedly improving wound healing of various tissues by utilizing undifferentiated autologous host cells.  While stem cells may be isolated from various locations in the human body, more recently it has been shown that low levels of mesenchymal stem cells also exist circulating within peripheral blood. Platelet rich fibrin (PRF) is a regenerative modality that utilizes peripheral blood + centrifugation protocols without the use of anti-coagulants to create a three-dimensional tissue engineering scaffold containing both growth factors and autologous cells. Very recently, it has been shown that modifications to centrifugation speed and time following recently developed concepts (the low-speed centrifugation concept or LSCC) resulted in a marked increase in host cells and growth factors. Within these scaffold constructs, mesenchymal stems cells were also found following collection with this relatively painless and low-cost modality. The objective of the present talk will be to present recent modifications to centrifugation speed and time to optimize stem cell quantities within PRF. Thereafter, the biological data supporting their numbers, as well as their potential for clinical applications will be presented with data coming from many fields of medicine including for the regeneration of osteoarthritic knees, dental regenerative medicine, orthopaedic grafting, and for facial aesthetics.

Biography:

Abstract:

Introduction: The versatility and refi nement of biomaterials and tissues engineered for regenerative medicine is growing, as is the need to characterize their properties and host interactions. The topography and mechanical properties of biomaterials are crucial parameters that infl uence cell adhesion/motility, morphology and mechanics as well as the fate of stem and progenitor cells [1, 2, 3]. Methods: Atomic force microscopy (AFM) is a powerful tool which allows the comprehensive study of all these properties and interactions with nanometer scale resolution under controlled environmental conditions. The inherent drawbacks of traditional AFM imaging modes for fast imaging or for challenging samples like living cells has been impressively overcome by the novel NanoWizard® ULTRA Speed AFM, which not only enables high-speed studies of time-resolved dynamics associated with cellular processes, it’s latest scanner technologies and compact design also allow full integration of AFM into advanced commercially available light microscopy techniques. Thus, fast AFM imaging of several frames per second can be seamlessly combined with methods such as epi-fl uorescence, confocal, TIRF, STED microscopy, and many more. Results: Using AFM, mechanical properties like the Young’s modulus of biomaterials, tissues or cells can be determined. Furthermore, the nanostructure of biomaterials like aligned collagen matrices and cell alignment on such structures have been resolved [3]. Using Single Cell Force Spectroscopy (SCFS), cell-substrate or cell-cell/tissue interactions can be measured down to single protein unbinding. The Nano-mechanical analysis of cells is increasingly gaining in importance in different fi elds in cell biology like cancer research and developmental biology. Discussion & Conclusions: We will present how the latest advances in the ULTRA Speed AFM are being applied to studyt a wide-range of biological specimen, from individual biomolecules to collagen type I fi brillogenesis to mammalian cells and tissues.

Biography:

Omid Panahi, Graduated from Centro Escolar University on 2013 in fi eld of Doctor of Dental Mediciene( DMD), and MSc Oral and maxillofacial surgery at yeditepe university, Istanbul, turkey. He has published more than 40 papers in reputed journals and has been serving as an editorial board member of isi journals.

Abstract:

Stem cells are unique cells and reproducible cells that can be useful as a good alternative to future therapies in the fi eld of Medicine. Some body cells, including blood cells and nerve cells, cannot be replaced, and stem cells play an important role in this procedure, which can help to replace cells, while stem cells can be used as alternate cells several times, and this act of replacing damaged cells called proliferation. In this paper, I tried to investigate the role of stem cells in regenerative medicine. Since Stem cell-based Regenerative medicine is one of the new and effective branches, it should have wider research on stem cells in order to be more effective in medical and biological sciences.

Biography:

M.V. Kovina graduated with honorary degree from the biological department of M.V.Lomonosov’s Moscow State University in 1988 and received there her PhD in biochemistry (1998). In 2010 Marina Valentinovna founded the 1st Russian Longevity School. Since 2014 Marina Valentinovna has been working at Sechenov’s First Moscow State Medical University. She has published 30 papers in reputed journals and was awarded the European Academia prize for young scientists of NIS and the George Soros foundation prize (1998), the Glenn award (2007) and the Moscow Region Governor prize for the best social project in the nomination “The Third Age” (2014).

Abstract:

The increase in MLS, maximum lifespan, is the most signifi cant indicator of affecting the basic mechanisms of aging, in particular, the age-related loss of stem cells. The transplantation of young bone marrow (BM) to laboratory mice of advanced age, results in 6-30% of average life span extension in varies studies, while we achieved 31+-3% extension of maximal life span due to nonablative syngeneic large infusion technology. The survival time from the beginning of the experiment increased 3+-0.3-fold. The chimerism of the bone marrow 6 months after the transplantation was 28%. The result is encouraging for clinical adaptation for aged humans (70-80-years old). The richest source of highly proliferative human mesenchymal stem cells is menstrual blood. We have developed the high yield isolation approach resulting up to four million nucleated cells per milliliter of initial blood, of which about 0.2-0.3% are colony-forming cells expressing standard mesenchymal markers CD90, CD105, and CD73, but not expressing CD45, CD34, CD117, CD133, or HLA-G. The cells have high proliferative potential (doubling in 26 h) and the ability to differentiate into adipocytes and osteocytes. Early endometrial MSCs (eMSCs) express epithelial marker cytokeratin 7 (CK7). We show for the fi rst time that a satisfactory and stable yield of eMSCs is observed throughout the whole menstrual period (fi ve consecutive days) of a healthy woman. eMSC mass cryobanking would solve the issue of donation, which is very acute now and might become even more complicated in the future, especially in view of geriatric application of stem cells.

Biography:

Despoina Kesidou has completed her BSc in Molecular Biology and Genetics in Democritus University of Thrace, studying: “The effects of cryopreservation on human biopsied embryos”. Following this, she completed her MSc in Nanotechnology and Regenerative Medicine at University College London, where she studied: “The development of novel antibody functionalized coronary artery stents for the promotion of endothelisation”.

Abstract:

Cardiovascular disease (CVD) is the leading cause of mortality worldwide claiming almost 17.7 million deaths annually. Despite much progress in stent development, unwanted side effects are yet to be resolved. Since the main cause is poor endothelisation surrounding damaged vessels, there is increasing need for novel approaches to enhance endothelialization. We have developed a simple approach using polycarbonate-urea urethane pre-polymers, which can be easily coated on to metallic biomaterials such as 316L stainless steel and chemically modifi ed to produce amine groups. These groups can be exploited for site directed immobilization of monoclonal antibodies such as CD34+, CD31+, CD133+ that bind to endothelial cell receptors. Surface modifi cation was verifi ed using contact angle (θ°), ATRFTIR, ToF-SIMS, orange II assay and ELISA. EC based assays used Alamarblue®, total DNA and immunohistochemical staining to study the effect of these platforms on human umbilical vein endothelial cells. Our results demonstrate that antibody immobilization signifi cantly increased cell metabolic activity and proliferation (p<0.05). CD34 antibody immobilization resulted in an increase of ~54% in metabolic activity and ~62% in total DNA, the immobilization of CD31 antibody to ~60% increased metabolic activity and ~67% increased DNA, while CD133 immobilization led to an increase of ~56% in metabolic activity and ~63% in total DNA when compared with controls. Moreover, co-immobilization of an antibody mixture on a single platform resulted in ~65% increase in HUVEC metabolic activity and ~70% increase in total DNA. These results confi rmed that our approach can promote endothelisation in vitro, and current studies will explore their infl uence on in-stent restenosis and thrombosis.